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LI-COR
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ProMab Inc
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EpiGentek
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ZSGB Biotech
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Biogenex
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Amresco
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Abmart Inc
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Image Search Results
Journal: Experimental and Therapeutic Medicine
Article Title: Effects of bone marrow-derived mesenchymal stem cells on the autophagic activity of alveolar macrophages in a rat model of silicosis
doi: 10.3892/etm.2016.3200
Figure Lengend Snippet: Western blot analysis showing Beclin-1 protein expression. (A) Western blot bands of Beclin-1 and (B) densitometry analysis of the Beclin-1 band corresponding to β-actin. ODs of the respective protein bands were analyzed using Image J software. Bars represent the mean ± standard error of the mean (n=5 per group). *P<0.05, vs. control group; #P<0.05, vs. model group. Beclin-1, autophagy-related gene Beclin-1; BMSC, bone marrow-derived mesenchymal stem cell; OD, optical density.
Article Snippet: Following blocking, the membranes were incubated overnight at 4°C with polyclonal rabbit anti-LC3 (1:500) and anti-Beclin-1 (1:200) antibodies and a monoclonal mouse (074–1806; 1:5,000; KPL, Inc.)
Techniques: Western Blot, Expressing, Software, Derivative Assay
Journal: Experimental and Therapeutic Medicine
Article Title: Effects of bone marrow-derived mesenchymal stem cells on the autophagic activity of alveolar macrophages in a rat model of silicosis
doi: 10.3892/etm.2016.3200
Figure Lengend Snippet: Western blot analysis showing LC-3 protein expression. (A) Western blot bands of LC-3 and (B) densitometry analysis of the LC-3 band corresponding to β-actin. Bars represent the mean ± standard error of the mean (n=5 per group). *P<0.05, vs. control group; #P<0.05, vs. model group. LC-3, microtubule-associated protein light chain 3; BMSC, bone marrow-derived mesenchymal stem cell; OD, optical density.
Article Snippet: Following blocking, the membranes were incubated overnight at 4°C with polyclonal rabbit anti-LC3 (1:500) and anti-Beclin-1 (1:200) antibodies and a monoclonal mouse (074–1806; 1:5,000; KPL, Inc.)
Techniques: Western Blot, Expressing, Derivative Assay
Journal: Biochemistry and Biophysics Reports
Article Title: An appropriate loading control for western blot analysis in animal models of myocardial ischemic infarction
doi: 10.1016/j.bbrep.2017.09.001
Figure Lengend Snippet: Protein levels of β-tubulin, β-actin and GAPDH in the heart tissue from animal model of myocardial ischemia. (A, B) Western blot and quantitative analysis of β-tubulin, β-actin and GAPDH protein levels in heart tissue from Rhesus monkey model of early stages of myocardial ischemia (2 h after surgery, n = 2; 24 h after surgery, n = 2). (C, D) Western blot and quantitative analysis of β-tubulin, β-actin and GAPDH protein levels in heart tissue from Rhesus monkey model of myocardial infarction (4 months after surgery, n = 4). (E, F) Western blot and quantitative analysis of β-tubulin, β-actin and GAPDH protein levels in heart tissue from mouse model of different time point (1 d after surgery, n = 6; 4 d after surgery, n = 6; 7 d after surgery, n = 6). Sham: sham operated control (n = 8); IA: ischemic/ infarct area; RA: remote area. Blots were analyzed by densitometry using Fusion-Capt Software. Results are presented as means ± SD. Three independent experiments were run for each result. *: P < 0.05 versus sham operated control; #: P < 0.05.
Article Snippet: The blots were then incubated with primary antibodies anti-β-actin, GAPDH, and
Techniques: Animal Model, Western Blot, Control, Software